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ktrA [2017-04-10 10:42:59]
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ktrA [2017-04-10 10:42:59]

high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
locus
BSU31090
pI
5.98
mw
24.73 kDa
protein length
222 aa Sequence Blast
gene length
666 bp Sequence Blast
function
potassium uptake
product
high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
essential
no
synonyms
yuaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|YdaO riboswitch]

    • Gene

    Coordinates
    3,188,414 -> 3,189,082

    Phenotypes of a mutant

  • The ''[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]'' mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) [Pubmed|16321950]

    • The protein

    Paralogous protein(s)

  • [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]

    • [SW|Domains]

  • contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK N-terminal domain* UniProt])
  • contains a [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK C-terminal domain* UniProt])

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex) [Pubmed|23598340]
  • [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 48% identity) [Pubmed|25957408]
  • [PDB|1LSU] (complex with NADH)
  • [http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)

    • [SW|Localization]

  • peripheral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]
    description
    [Pubmed|12562800]

    regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP, in [regulon|ydaO riboswitch|ydaO riboswitch]
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    Biological materials

    Mutant

  • MGNA-B543 (yuaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1542 NBRP B. subtilis, Japan]
  • 1A954 ( ''ktrA''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
  • GHB1 (D(''[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]'')::''aphA3''), available in [SW|Erhard Bremer]'s lab
  • GP92 (D(''[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP2083 (D(''[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]'')::''aphA3'' D''[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • GP2176 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
  • GP2177 (based on [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 25869574,27935846,28086088

    • Original publications

  • 12562800,15096624,16321950,20511502,23086297,23598340,24141192,25957408,16990138,26771197,28086091